Population Structure of Phytophthora plurivora on Rhododendron in Oregon Nurseries.
Identifieur interne : 000413 ( Main/Exploration ); précédent : 000412; suivant : 000414Population Structure of Phytophthora plurivora on Rhododendron in Oregon Nurseries.
Auteurs : Nicholas C. Carleson [États-Unis] ; Valerie J. Fieland [États-Unis] ; Carolyn F. Scagel [États-Unis] ; Jerry E. Weiland [États-Unis] ; Niklaus J. Grünwald [États-Unis]Source :
- Plant disease [ 0191-2917 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Espaceur de l'ADN ribosomique, Phytophthora.
- parasitologie : Maladies des plantes, Rhododendron.
- physiologie : Phytophthora.
- Orégon.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Ribosomal Spacer.
- geographic : Oregon.
- classification : Phytophthora.
- genetics : Phytophthora.
- parasitology : Plant Diseases, Rhododendron.
- physiology : Phytophthora.
Abstract
Phytophthora plurivora is a recently described plant pathogen, formerly recognized as P. citricola. Recent sampling of Pacific Northwest nurseries frequently encountered this pathogen, and it has been shown to be among the most damaging Phytophthora pathogens on ornamentals. We characterized the population structure of P. plurivora in a survey of four Oregon nurseries across three different counties with focus on Rhododendron hosts. Isolates were identified to the species level by Sanger sequencing and/or a PCR-RFLP assay of the internal transcribed spacer (ITS) region. We used genotyping-by-sequencing to determine genetic diversity. Variants were called de novo, resulting in 284 high-quality variants for 61 isolates after stringent filtering. Based on Fst and AMOVA, populations were moderately differentiated among nurseries. Overall, population structure suggested presence of one dominant clonal lineage in all nurseries, as well as isolates of cryptic diversity mostly found in one nursery. Within the clonal lineage, there was a broad range of sensitivity to mefenoxam and phosphorous acid. Sensitivity of the two fungicides was correlated. P. plurivora was previously assumed to spread clonally, and the low genotypic diversity observed within and among isolates corroborated this hypothesis. The broad range of fungicide sensitivity within the P. plurivora population found in PNW nurseries has implications for managing disease caused by this important nursery pathogen. These findings provide the first perspective into P. plurivora population structure and phenotypic plasticity in Pacific Northwest nurseries.
DOI: 10.1094/PDIS-12-18-2187-RE
PubMed: 31140922
Affiliations:
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Grünwald</name><affiliation wicri:level="2"><nlm:affiliation>2Horticultural Crops Research Unit, United States Department of Agriculture, Agricultural Research Service, Corvallis, OR.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Oregon</region></placeName><wicri:cityArea>2Horticultural Crops Research Unit, United States Department of Agriculture, Agricultural Research Service, Corvallis</wicri:cityArea></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Ribosomal Spacer (genetics)</term><term>Oregon (MeSH)</term><term>Phytophthora (classification)</term><term>Phytophthora (genetics)</term><term>Phytophthora (physiology)</term><term>Plant Diseases (parasitology)</term><term>Rhododendron (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Espaceur de l'ADN ribosomique (génétique)</term><term>Maladies des plantes (parasitologie)</term><term>Orégon (MeSH)</term><term>Phytophthora (classification)</term><term>Phytophthora (génétique)</term><term>Phytophthora (physiologie)</term><term>Rhododendron (parasitologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Oregon</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Espaceur de l'ADN ribosomique</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term><term>Rhododendron</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Rhododendron</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Orégon</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Phytophthora plurivora</i> is a recently described plant pathogen, formerly recognized as <i>P. citricola</i>. Recent sampling of Pacific Northwest nurseries frequently encountered this pathogen, and it has been shown to be among the most damaging <i>Phytophthora</i> pathogens on ornamentals. We characterized the population structure of <i>P. plurivora</i> in a survey of four Oregon nurseries across three different counties with focus on <i>Rhododendron</i> hosts. Isolates were identified to the species level by Sanger sequencing and/or a PCR-RFLP assay of the internal transcribed spacer (ITS) region. We used genotyping-by-sequencing to determine genetic diversity. Variants were called de novo, resulting in 284 high-quality variants for 61 isolates after stringent filtering. Based on <i>F</i><sub>st</sub> and AMOVA, populations were moderately differentiated among nurseries. Overall, population structure suggested presence of one dominant clonal lineage in all nurseries, as well as isolates of cryptic diversity mostly found in one nursery. Within the clonal lineage, there was a broad range of sensitivity to mefenoxam and phosphorous acid. Sensitivity of the two fungicides was correlated. <i>P. plurivora</i> was previously assumed to spread clonally, and the low genotypic diversity observed within and among isolates corroborated this hypothesis. The broad range of fungicide sensitivity within the <i>P. plurivora</i> population found in PNW nurseries has implications for managing disease caused by this important nursery pathogen. These findings provide the first perspective into <i>P. plurivora</i> population structure and phenotypic plasticity in Pacific Northwest nurseries.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31140922</PMID><DateCompleted><Year>2019</Year><Month>08</Month><Day>09</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>103</Volume><Issue>8</Issue><PubDate><Year>2019</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>Population Structure of <i>Phytophthora plurivora</i> on <i>Rhododendron</i> in Oregon Nurseries.</ArticleTitle><Pagination><MedlinePgn>1923-1930</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-12-18-2187-RE</ELocationID><Abstract><AbstractText><i>Phytophthora plurivora</i> is a recently described plant pathogen, formerly recognized as <i>P. citricola</i>. Recent sampling of Pacific Northwest nurseries frequently encountered this pathogen, and it has been shown to be among the most damaging <i>Phytophthora</i> pathogens on ornamentals. We characterized the population structure of <i>P. plurivora</i> in a survey of four Oregon nurseries across three different counties with focus on <i>Rhododendron</i> hosts. Isolates were identified to the species level by Sanger sequencing and/or a PCR-RFLP assay of the internal transcribed spacer (ITS) region. We used genotyping-by-sequencing to determine genetic diversity. Variants were called de novo, resulting in 284 high-quality variants for 61 isolates after stringent filtering. Based on <i>F</i><sub>st</sub> and AMOVA, populations were moderately differentiated among nurseries. Overall, population structure suggested presence of one dominant clonal lineage in all nurseries, as well as isolates of cryptic diversity mostly found in one nursery. Within the clonal lineage, there was a broad range of sensitivity to mefenoxam and phosphorous acid. Sensitivity of the two fungicides was correlated. <i>P. plurivora</i> was previously assumed to spread clonally, and the low genotypic diversity observed within and among isolates corroborated this hypothesis. The broad range of fungicide sensitivity within the <i>P. plurivora</i> population found in PNW nurseries has implications for managing disease caused by this important nursery pathogen. These findings provide the first perspective into <i>P. plurivora</i> population structure and phenotypic plasticity in Pacific Northwest nurseries.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Carleson</LastName><ForeName>Nicholas C</ForeName><Initials>NC</Initials><AffiliationInfo><Affiliation>1Department of Botany & Plant Pathology, Oregon State University, Corvallis, OR.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fieland</LastName><ForeName>Valerie J</ForeName><Initials>VJ</Initials><AffiliationInfo><Affiliation>1Department of Botany & Plant Pathology, Oregon State University, Corvallis, OR.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scagel</LastName><ForeName>Carolyn F</ForeName><Initials>CF</Initials><AffiliationInfo><Affiliation>2Horticultural Crops Research Unit, United States Department of Agriculture, Agricultural Research Service, Corvallis, OR.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Weiland</LastName><ForeName>Jerry E</ForeName><Initials>JE</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-1431-1702</Identifier><AffiliationInfo><Affiliation>2Horticultural Crops Research Unit, United States Department of Agriculture, Agricultural Research Service, Corvallis, OR.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grünwald</LastName><ForeName>Niklaus J</ForeName><Initials>NJ</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1656-7602</Identifier><AffiliationInfo><Affiliation>2Horticultural Crops Research Unit, United States Department of Agriculture, Agricultural Research Service, Corvallis, OR.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009922" MajorTopicYN="N" Type="Geographic">Oregon</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="Y">Phytophthora</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029793" MajorTopicYN="Y">Rhododendron</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N"></Keyword><Keyword MajorTopicYN="N">mycology</Keyword><Keyword MajorTopicYN="N">oomycetes</Keyword><Keyword MajorTopicYN="N">ornamentals</Keyword><Keyword MajorTopicYN="N">population genetics</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>8</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31140922</ArticleId><ArticleId IdType="doi">10.1094/PDIS-12-18-2187-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><country name="États-Unis"><region name="Oregon"><name sortKey="Carleson, Nicholas C" sort="Carleson, Nicholas C" uniqKey="Carleson N" first="Nicholas C" last="Carleson">Nicholas C. Carleson</name></region><name sortKey="Fieland, Valerie J" sort="Fieland, Valerie J" uniqKey="Fieland V" first="Valerie J" last="Fieland">Valerie J. Fieland</name><name sortKey="Grunwald, Niklaus J" sort="Grunwald, Niklaus J" uniqKey="Grunwald N" first="Niklaus J" last="Grünwald">Niklaus J. Grünwald</name><name sortKey="Scagel, Carolyn F" sort="Scagel, Carolyn F" uniqKey="Scagel C" first="Carolyn F" last="Scagel">Carolyn F. Scagel</name><name sortKey="Weiland, Jerry E" sort="Weiland, Jerry E" uniqKey="Weiland J" first="Jerry E" last="Weiland">Jerry E. Weiland</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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